Thermo Scientific gibco CTS Rotea User Manual page 84

Chapter 4 Protocol Building Blocks
4
Elutriation
(continued)
Note: The elutriation break points can be quite a sharp step change, with subtle changes in speed
having a significant impact on the purity and rate of elutriation. Determining and optimizing these break
points may take several iterations.
Integrate elutriation and cell concentration functions
™
The Rotea instrument enables cells that have been separated through elutriation to then be
concentrated as part of an automated process.
In some cases, the target cells are retained in the fluidized bed and can simply be recovered as
concentrate.
When the target cells are being elutriated from the chamber, they can be directed to a dual port
intermediate bag through line G. This leaves the concentrated non-target cells in the chamber which
can be recovered to ports E, F or H.
The elutriated target cell product can now be loaded into the CFC Chamber through port D, washed if
required, and delivered as concentrate to port E, F or H.
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CTS ™ Rotea
To determine the elutriation volume required to
achieve the required purity:
• Load the PBMC into the bed and elutriate at the
speed for lymphocyte separation.
• Take samples from the sampling port every
50 mL or so to determine when the lymphocytes
stop emerging.
™ Counterflow Centrifugation System Process Design User Guide
Details