Maximize Throughput - Thermo Scientific gibco CTS Rotea User Manual

Maximize throughput

Some processes eg post expansion, require a significant volume of input material to be processed
within a limited time frame. In counter flow centrifugation, processing at high G force also means
running at a higher flow rate to sustain the fluidized bed. The Rotea
Single-Use Kit option for cell and media combinations that allow it.
The optimum flow rate for a given process will be a balance of throughput and yield determined from
process development runs.
Having determined the optimum process flow rate, the number of cells that can be retained within the
fluidized bed can be estimated through experimentation and from this, the volume per bite that can be
loaded into the CFC Chamber.
With smaller cells e.g. 6 to 8 μm T-cells, a significant time per bite can be consumed in establishing the
bed. One approach is to not recover the full cell population after processing each bite. Establishment
of the subsequent bed is then commenced using the residual cells from the prior bite to accelerate bed
formation.
• The loading speed for a stable fluidized bed is affected by the size of the bed, with higher flow rates
possible as the bed increases in size. Slowly increasing the flow rate as the fluidized bed builds can
reduce the total processing time.
• The Concentrate Recovery step is very fast compared to loading. It may be better to do more
bites at higher flow rates with less cells per bite than to load at a very low flow rate and create a
highly concentrated bed of cells.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
Chapter 4 Protocol Building Blocks
Multi-bite processing
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instrument also has a high flow
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