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Chapter 5 Rotea
™
Process Model
5
Predict process settings
The net force on the particle is plotted in purple. Where the net force is >0, the particles are
driven inwards towards the axis of rotation by the Stokes drag rather than settling under centrifugal
acceleration. Where the net force is below the line, the particles will settle towards the tip of the
chamber.
Examples of fluidized beds highlight predicted behavior. The left image is of beads. The very clear zone
in the tip clearly reflects the expected clear zone highlighted by the red zone in the chart. The image on
the right is CHO cells with a well-formed bed.
Note: The tip of the cone is not clear in this case suggesting the flow rate could be higher.
Alternatively, there may be larger cells in the population that are settling at the tip while the smaller
cells are in the fluidized bed.
Predict process settings
™
The Rotea
Process Model is based on the theoretical model, see "Counter flow centrifugation forces"
on page
96, including several assumptions.
In the absence of proven settings from a prior protocol, the Rotea
to estimate the flow rate and g force required to either concentrate or elutriate different cell types in
various media.
To open the Rotea
16 and click on
98
™
Process Model, select the protocol step number that you wish to model e.g. Step
in the top right corner of the Process Model home screen.
CTS
™
™
Rotea
™
Counterflow Centrifugation System Process Design User Guide
Process Model allows the user
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