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Prime Large Bore Line
Draw the air brought into the Bubble Sensor down into the Bubble Trap or out into another bag. All
settings should remain the same as the previous step, except using a Volume trigger instead of a
Bubble Sensor Input trigger. The trigger volume should be calculated using the Priming Volumes
table to ensure any air not caught in the bubble trap travels all the way into the waste bag. In this
example (D to A) we use a volume of 5 mL.
Manage air in the system
When first priming it is possible to shift air around the Single-Use Kit with the peristaltic pump. If, for
example, the output bag is destined for cryo-storage it is usually preferred that no air is in the bag once
filled. In this situation use the method shown above.
Establish a fluidized bed
Overview
A stable fluidized bed is the central concept that counterflow centrifugation and Rotea
is built around. The most essential part of creating a good protocol using the Rotea
understanding how to establish a good fluidized bed.
Example of fluid path through the CFC Chamber:
CTS
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Rotea
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Counterflow Centrifugation System Process Design User Guide
Chapter 4 Protocol Building Blocks
Establish a fluidized bed
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instrument
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instrument is
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