Basic Priming Steps - Thermo Scientific gibco CTS Rotea User Manual

Basic priming steps

A typical priming sequence consists of 9 steps, as shown in this example:
Note: The priming sequence will always be protocol dependent. It is the users responsibility to
determine the priming steps that are appropriate to their specific application, the basic steps and
settings shown below are recommended.
Pre-Prime
The Pre-Prime step is used to ensure fluid has entered the Single-Use Kit regardless of the length of
tube that connects to the bag containing the priming fluid. To achieve this, the Bubble Sensor Input
trigger is set Dry to Wet and hence will detect the arrival of fluid at the Bubble Sensor. The centrifuge
should be at 0 G (not yet spinning), and the pump should be at a high flow rate (100 mL).
A typical option for the Pre-Prime step is from your wash buffer to your waste bag – B to A in this
example.
Lubricate Rotary Coupling
After the Bubble Sensor goes off and advances to the next step, the pump delivers 15 mL of priming
fluid through the system before starting centrifuge rotation. The centrifuge should be set to 0 G and with
a high pump flow rate e.g. 100 mL/min.
The fluid path is the same as the Pre-Prime step – in this example, B to A.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
Chapter 4 Protocol Building Blocks
4
Priming
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