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Chapter 4 Protocol Building Blocks
4
Elutriation
Elutriation techniques
™
The Rotea
instrument provides the tools to develop and optimize elutriation processes and settings.
Lines A and G of the Single-Use Kit direct elutriated material (media and cells) to either a waste bag or
intermediate bag for subsequent processing.
Note: If line E or F is being used as the input, it is also possible to direct elutriated material through the
Bubble Trap from the left and out via lines B, C or D.
When developing an elutriation method, the challenge is to understand:
• Which cells am I trying to retain in the fluidized bed and which cells am I trying to elutriate?
• At what "speed" do the cells I want to elutriate start to leave the CFC Chamber?
• How long do I need to elutriate at a given "speed" to achieve an acceptable level of purity?
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CTS
™
Rotea
™
Counterflow Centrifugation System Process Design User Guide
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