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4
Priming
Overview
The first steps in any protocol are used to prime the system. Priming has two main functions:
• lubricate the Rotary Coupling
• replace air in the Single-Use Kit with fluid
It is essential to prime any line through which fluid will enter the system. Any further priming of lines is
dependent upon the specific protocol.
WARNING! Insufficient wetting of the Rotary Coupling can lead to premature failure of the Single-
Use Kit. Whilst recommended priming steps and settings are described in this section of the user
guide, adequate wetting is ultimately dependent on the media used, the pump flow rate, centrifuge G
force settings and the protocol itself.
Bubbles
It is important to remove all air from the system. If bubbles enter the process line feeding the centrifuge
at operating speed, they accumulate within the Rotary Coupling causing the pressure to rapidly
increase. To protect the integrity of the Single-Use Kit, the instrument will detect this over pressure
condition through the Pressure Sensor and trigger an automatic stop.
CTS
™
Rotea
™
Counterflow Centrifugation System Process Design User Guide
Protocol Building Blocks
47
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