Cell Loading; Overview; Cells Loaded Into The Cfc Chamber - Thermo Scientific gibco CTS Rotea User Manual

Chapter 4 Protocol Building Blocks
4

Cell loading

Cell loading

Overview

Cell loading is the process of capturing target cells in the fluidized bed as the input material passes
through the CFC Chamber.

Cells loaded into the CFC Chamber

The CFC Chamber has a fluidized bed volume of 10 mL. Due to its conical shape, a small volume of
cells represents a reasonable size bed as can be shown in the following illustration:
The number of cells that can be processed in the CFC Chamber at a time is dependent on the
following:
• Size and concentration of the cells
• Density of the media
• G force and flow rate settings that will deliver acceptable cell recovery and viability
There are two practical limitations to the number of cells in the bed that can be determined through
experimentation:
• When the top surface of the bed extends past the CFC Chamber rim closest to the axis of rotation
(ie >10 mL), cells will be washed out of the CFC Chamber
• When the fluidized bed is too concentrated to recover
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide