Thermo Scientific gibco CTS Rotea User Manual page 51

Chapter 4 Protocol Building Blocks
4
Priming
Ramp to next step
The next step after priming will often require a significantly higher centrifuge speed and a change in
the pump flow rate. It is preferable to insert a pause step with speed ramp immediately after priming
to enable the user to check that priming has been successfully achieved, provide an opportunity for
residual air within the CFC Chamber to be released and stabilize the system at the new speeds in a
controlled manner. In the example above we have created a 10 second pause step with a 5 second
ramp time.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide