The Seven Parameters For Establishing A Fluidized Bed - Thermo Scientific gibco CTS Rotea User Manual

The seven parameters for establishing a fluidized bed

The theoretical model and key process parameters that define the behaviour of cells in the CTS
™
Rotea Counterflow Centrifuge is described in the formula below (see Chapter 5, "Rotea
Model" for further details).
Seven parameters affect formation of a fluidized bed to varying degrees and need to be considered
when developing a protocol.
1. Cell diameter and cell density are fixed for the cell type.
2. G force and flow rate are readily adjustable settings in the CTS
Centrifugation System.
3. Density and viscosity of media can sometimes be optimized to suit the protocol.
4. Cell concentration is not considered in the formula but has a significant influence on the ability of
the bed to form.
5. The conical shape of the chamber has been specifically designed such that the increasing cross-
sectional area of the chamber varies the flow rate of the media as it passes through the chamber.
This counteracts the change in centripetal force due to the radial position of the particle.
™
6. The Rotea
conditions.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
Process Model uses this formula to predict the behavior of cells under different
Chapter 4 Protocol Building Blocks
Establish a fluidized bed
™
™ ™
Rotea Counterflow
4
™
Process
55