Thermo Scientific gibco CTS Rotea User Manual page 26

Chapter 3 Rotea Protocol Builder Application
3
Step view
1
Description: Enter a description for your step. Keep
your descriptions clear and concise to enable the user
to quickly comprehend what each step does. This
description will also be displayed in the List view.
2
Choose Step Type: Choose the step type from the
options in the dropdown menu.
3
Trigger: Define the triggers that will be used to
advance to the next step.
Note: Multiple triggers can be selected for each step.
The first trigger reached will cause the protocol to
advance.
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4
Centrifuge: Enter the desired G force to be generated
by the centrifuge.
5
Pump: Enter the pump speed in mL/min
6
Ramp Time: When the ramp time is set to zero, default
speed ramps will be applied to the change in G force
and pump speed between steps. When there is a
significant difference in speeds between steps, it may
be desirable to ramp speeds more gradually to ensure
a smooth transition. Inserting a ramp time will cause
both the G force and pump speed to change to their
new speed settings over the specified time.
Note: Ramp time must be less than or equal to the
step time.
7
• Click the Skip Button check box if you wish to
enable the ADVANCE button on the instrument so
that the user can manually skip to the next step
during operation of the protocol.
Note: The default for the Skip Button is off. The
ADVANCE function on the instrument is very useful
during process development, however, use with
caution on processes where manual intervention
needs to be restricted e.g. qualified processes. The
skip button can also be used as a step trigger.
• Click the Set as Last Step checkbox to have the
protocol end after the step has run.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide