Pause Step - Thermo Scientific gibco CTS Rotea User Manual

Pause step

A recommended strategy is to include a brief (5 to 10 second) Pause step prior to Concentrate
Recovery to adjust the speeds and hence concentration of the fluidized bed. This provides several
benefits:
• The Pause step actuates valves that may not have been recently used but will be important during
Concentrate Recovery
• It is a convenient step from which to restart a protocol after an interruption or stoppage
• Concentrate recovery typically happens directly after washing where it may have been necessary to
concentrate the bed to minimize disturbance of the fluidized bed. It can be advantageous to slightly
expand the bed and flush compacted cells from the tip of the cone prior to Concentrate Recovery
• Adding a Speed Ramp to the Pause step provides a smooth speed transition to expand or
concentrate the bed without loss of cells.
• If you are using a Harvest step the Pause loop is the ideal destination for fluid prior to the valve
changeover.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
Chapter 4 Protocol Building Blocks
Concentrate Recovery
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