Thermo Scientific gibco CTS Rotea User Manual page 54

Chapter 4 Protocol Building Blocks
4
Establish a fluidized bed
Media containing both large and small cells enters the Rotary Coupling via the input line and flows into
the CFC Chamber, exiting at the tip of the stainless-steel Dip Tube.
The media immediately changes direction and flows back towards the axis of rotation.
As the media passes through the CFC Chamber, larger cells are loaded to the fluidized bed whilst
smaller cells are elutriated and exit the CFC Chamber via the output.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide