Thermo Scientific gibco CTS Rotea User Manual page 61

Chapter 4 Protocol Building Blocks
4
Establish a fluidized bed
Fluidized bed formation
Image Description
In the early stages of establishing a bed, cells
can be seen entering the chamber through the tip,
and flowing up the top edge of the chamber, a
consequence of Coriolis flow.
As more cells enter the chamber the bed begins to
stabilize. The cells are still affected by Coriolis flow at
this point, but the angle of the bed is getting closer
to vertical.
The number of cells in the chamber has increased
and we can see that the bed is nearly vertical with
minimal turbulence.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide