Thermo Scientific gibco CTS Rotea User Manual page 34

Chapter 3 Rotea Protocol Builder Application
3
Trigger
Volume Register
Selecting Use Volume Register allows you to select any valid variable from a dropdown menu
including:
• Variables defined in the Data Entry screen
• Valves that are being used within the protocol.
• OD Sensor volume if a harvest step type is being used
Note: The volume used for a valve trigger is the absolute value of the volume that has passed
through the valve over the course of the protocol. Flow into a bag is tracked as a positive and flow
out of a bag is tracked as negative.
You can also apply a multiplier to the volume trigger.
A volume register trigger will advance to the next step when the volume pumped during the step equals
the volume in the Volume Register at the beginning of the step multiplied by the multiplier.
Note: All types of volume triggers are measured from the pump, not the bag. Setting a volume of
30 mL from B to A does not guarantee that 30 mL will end up in A, just that 30 mL will travel through the
pump.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide