Quality Control Of Ion Sphere ™ Particles; Quality Control Using The Guava; Maintain The Ion Chef ™ System - Thermo Scientific Ion 520 User Manual

When the instrument reset run completes, the instrument automatically performs the cleaning
procedure. After cleaning, the touchscreen returns to the main menu.
Quality control of Ion Sphere

Quality control using the Guava

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The Guava easyCyte 5 Benchtop Flow Cytometer can be used for quality assessment of unenriched
and enriched Ion Sphere
Sequencer, Ion S5
• Unenriched sample – Obtain the QC sample from the Library Sample Tube on the Ion S5
Chef Reagents cartridge at position A.
• Enriched sample – Obtain enriched sample from the tube on the Enrichment Cartridge v2 at
position E.
For details, see the Ion Sphere
Using the Guava
available at thermofisher.com.
Maintain the Ion Chef
For further information on the following Ion Chef
Instrument User Guide (Pub. No. MAN0018668), available at thermofisher.com.
• Install a firmware update
• Change the instrument name
• Replace the ultraviolet lamp
• Perform an XYƟ calibration
• Perform a Z calibration
Note: You must install the Ion Chef
later to access the XYƟ calibration and Z calibration routines.
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
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Particles generated for up to 400 to 600 base-read sequencing on an Ion S5
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XL Sequencer, or Ion GeneStudio
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Particles Quality Assessment for the Ion Proton
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easyCyte 5 Benchtop Flow Cytometer User Bulletin (Pub. No. MAN0007496),
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System
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Instrument firmware update in Torrent Suite
Appendix B Supplemental procedures
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Particles
easyCyte 5 Benchtop Flow Cytometer
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S5 Series Sequencer.
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System maintenance procedures, see the Ion Chef
Quality control of Ion Sphere ™
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and Ion S5
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Software 5.12 or
B
Particles
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ExT
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Systems
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