Thermo Scientific Ion 520 User Manual page 44

Chapter 4 Start the Ion Chef
4
Unload the chip for sequencing
5. Load the chip into a sequencer, then promptly start the sequencing run.
If you cannot sequence a loaded chip immediately or plan to sequence two chips per initialization,
place the chip into a separate chip storage container and store at 4°C until you are ready to
sequence it (up to 6–8 hours maximum).
IMPORTANT!
· Liquid may be present in chip wells after the Ion Chef
from the wells.
· If you choose to store a loaded chip, remove the chip from 4°C storage (but keep it in the
storage container) at least 20 minutes before running it, allowing the chip to warm to room
temperature.
44
™ run
™
run. Do NOT remove any residual liquid
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide