Thermo Scientific Ion 520 User Manual page 2

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Revision history Pub. No. MAN0015805
:
Revision
F.0
E.0
D.0
C.0
B.0
A.0
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Date
24 November 2021
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25 June 2019
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12 December 2018
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22 February 2017 Volume of Ion S5
changed from 24 µL to 16 µL.
26 January 2017
•
•
13 October 2016 New user guide with detailed instructions for use of the Ion 520
Chef with the Ion Chef
™
Updated "Start the Ion Chef
"Dilute the sample library" on page
™
Updated "Start the Ion Chef
control library" on page 25.
™
Updated for Torrent Suite Software 5.12.
Separated "Prepare the libraries and consumables" into two topics: "Prepare the
consumables" and "Add the diluted libraries to the Ion Chef
Tube".
™
Updated "Start the Ion Chef
step‑by‑step Ion Chef ™ run setup.
Updated the guidance for storing loaded chips before sequencing in "Unload the
chip for sequencing".
Added a note in "Start the Ion Chef
to NOT remove any residual liquid from chip wells after the Ion Chef
Removed Ion Chef ™ System maintenance procedures from "Maintain the
™
Ion Chef System".
Updated "Maintain the Ion Chef
System maintenance procedures in Ion Chef
Updated "Related documentation" to include the Ion Chef
Guide.
™
Updated for Ion GeneStudio
™
Updated for Torrent Suite Software 5.10; Planned Run creation topics
reorganized for ease of use.
Clarification made to dilute barcoded library pools, if used.
Added a new troubleshooting topic: "Planned Run status does not advance to
"Planned"".
Removed reference to quality control of ISPs using the Attune
Focusing Cytometer.
™
ExT Calibration Standard provided in ordered item (Cat. No. A30738)
Number of flows recommended for the instrument reset run (formerly "purge and
clean") procedure changed from 500 to 200, and recommended time to perform
the procedure after sequencer initialization changed from 27 hours to 24 hours
(see "Perform an instrument reset run with an initialized sequencer that is loaded
with an unused Reagents cartridge"
Web links updated
™
and the Ion S5
Description
run" to update the recommended concentration in
25.
run" to update the volume of library in "Dilute the
™
run" to include the Run Options screen for
™
run" and "Unload the chip for sequencing"
™
System" to add references to the Ion Chef
™ Instrument User Guide.
™
S5 Series Systems.
on page 66)
™ & Ion 530
™ ™
/Ion S5 XL Systems.
Library Sample
™ run.
™
Instrument User
™
NxT Acoustic
™ ExT Kit –