Interior Hardware And Consumables - Thermo Scientific Ion 520 User Manual

Interior hardware and consumables

The following figure illustrates the interior of the Ion Chef
involved in the preparation of chips for sequencing on an Ion S5
™
Ion GeneStudio
New pipette tips – The position of the rack containing unused pipette tips.
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Automated heated cover – Transfers the plate cover to the PCR reaction plate within the sample block. During
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thermal cycling, the heated cover applies compression to seal the reaction plate and heats the cover to prevent
condensation.
Waste pipette tip rack – The position of the rack containing waste (used) pipette tips.
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Thermal cycler sample block – Performs thermal cycling of the sequencing reactions on a 96-well PCR reaction
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plate.
Reagents station – The position on the instrument deck of the diluted libraries, NaOH, and the Ion S5
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Reagents cartridge.
Solutions station – The position on the instrument deck of the Ion S5
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at room temperature.
Recovery centrifuges – Twin stations that perform centrifugation of the ISPs during the recovery phase of template
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preparation.
Enrichment station – The position of the rack containing consumables for the enrichment of the template-positive
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ISPs.
Chip-loading centrifuge – Performs centrifugation of the sequencing chip that has been mounted to a chip-loading
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adapter and loaded with template-positive ISPs, and a chip balance loaded opposite to the sequencing chip.
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
S5 Series Sequencer.
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Chapter 1 Product information
About the Ion Chef
™
Instrument and describes the stations
™
Sequencer, Ion S5
™
Chef Solutions cartridge, which is maintained
1
™ System
™
XL Sequencer, or
™ ExT Chef
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