Thermo Scientific Ion 520 User Manual page 39

8. Ensure that the instrument displays the correct kit type, chip type, chip barcode, and Planned Run.
If the correct Planned Run does not display, tap the dropdown list
Planned Run, then tap Next.
IMPORTANT! If the kit and chip type are not correct, confirm that you are using the correct kit
and chip. If you are using the correct kit and chip, and an incorrect kit or chip type appears on the
screen, contact Technical Support.
9. On the Run Options screen, tap the appropriate option to complete the run, then enter the desired
time of run completion if needed.
The Ion Chef
can be used to evaluate templating efficiency. Depending on your selection, the QC sample will
be made available either during or after the run. In either case, you can obtain unenriched sample
from the Library Sample Tube at Position A on the Reagents cartridge, or enriched sample from
Position E on the Enrichment Cartridge v2.
By selecting
Time
Pause
[1]
An additional 1 hour is required for chip loading to complete the run. Total run time is longer because an extra centrifugation
step is required with the Pause option.
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
Balance Chip
No Selection
™
Instrument provides two options for obtaining a quality control (QC) sample that
You can obtain the QC samples
immediately after the run ends, at the time you
specify:
when the instrument pauses operation before the
chip loading step:
Chapter 4 Start the Ion Chef
Start the Ion Chef
to select the appropriate
Approximate time after run start
6.75 hours
5.75 hours
™ run
4
™
run
[1]
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