System; Load The Ion Chef - Thermo Scientific Ion 520 User Manual

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Load the Ion Chef

IMPORTANT!
·
Rated centrifuge speeds are intended for operation only with the provided buckets and approved
consumable chips, tubes, and sample preparation reagents.
·
The Chip-loading centrifuge is rated to operate at the listed rotational frequencies with the chip
buckets, chip, adapter, and chip balance. The centrifuge must be load-balanced. Proper care must
be taken to load the bucket properly. If excessive vibrations arise, check to ensure that items are
installed properly and rotors are load-balanced.
·
Use only the materials supplied in the Ion 520
the rated speeds. Do not remove or change the rotors. Inspect the buckets before each use to assure
normal operation.
·
Ensure that the instrument is powered on and has been cleaned following the last use.
·
Ensure that all components are clean and dry before loading them onto the Ion Chef
·
Ensure that the Reagents and Solutions station compartments are dry and free of condensate before
loading components.
Follow the procedure described in the following sections to load the Ion Chef
Figure 1 A schematic of a loaded Ion Chef
New Ion S5
ExT Tip Cartridge
1
Frame Seal v2 (inserted)
2
Empty tip rack (move from new Tip cartridge position)
3
PCR Plate
4
Ion S5
ExT Chef Reagents cartridge
5
Ion S5
Chef Solutions cartridge
6
Ion 520
& Ion 530
ExT Kit − Chef User Guide

System

1
2
3
4
5
6
7
8
9
10
11 12
A
B
C
D
E
F
G
H
Instrument.
Chapter 4 Start the Ion Chef
& Ion 530
ExT Kit – Chef to run the centrifuges at
Recovery centrifuges: Recovery Tubes and Recovery
7
Station Disposable Lid v2
Enrichment Cartridge v2
8
Chip-loading centrifuge: Chip Adapter/Chip assembly
9
and Chip Balance
run
Load the Ion Chef

System

Instrument.
Instrument.
4
27

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