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Thermo Scientific Nicolet iS50 User Manual
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Thermo Scientific Nicolet iS50 User Manual

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Nicolet iS50 Spectrometer
iS50 ATR Module
User Guide
269-277500 Revision A
June 2012

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Summary of Contents for Thermo Scientific Nicolet iS50

  • Page 1 Nicolet iS50 Spectrometer iS50 ATR Module User Guide 269-277500 Revision A June 2012...
  • Page 2 © 2012 Thermo Fisher Scientific Inc. All rights reserved. Windows is either a trademark or a registered trademark of Microsoft Corporation in the United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. For U.S.
  • Page 3 The optional iS™50 ATR module offers convenient, reproducible attenuated total reflection (ATR) sampling with the Nicolet™ iS50 spectrometer. Figure 1. Nicolet iS50 spectrometer with optional iS50 ATR module iS50 ATR module The iS50 ATR module mounts in the main cover of the spectrometer.
  • Page 4 ATR Module For information about additional sampling modules and accessories for your Nicolet iS50 spectrometer, contact our sales representative in your area. Contents • Important Features • Specifications • Compatible Software • Operating Precautions • Operation • Compatible Samples •...

  • Page 5: Important Features

    • ATR Touch Point. This button automatically configures the spectrometer for ATR sampling. The Touch Point LED shows the status of the ATR module. The LED has three states described below. Thermo Scientific iS50 ATR Module User Guide...

  • Page 6: Specifications

    1.5 Maximum sample thickness 12.7 mm (0.5 in) Useful pH range 1 to 14 Pressure arm maximum force 267 Newtons (60 lbs) Detector Dedicated broad-band DTGS (deuterated triglycine sulfate) iS50 ATR Module User Guide Thermo Scientific...

  • Page 7: Compatible Software

    • Make sure your sample will not react with the ATR crystal or crystal plate. Each material has unique characteristics. Please refer to the “Specifications” section for details. • Do not scrape the crystal with extremely hard materials such as corundum, or with a knife to remove particles. Thermo Scientific iS50 ATR Module User Guide...

  • Page 8: Operation

    (267 N or 60 lbs) is achieved and additional rotation of the control knob has no effect. This mechanism is designed to protect the crystal from damage. To raise the pressure arm, turn the control knob counterclockwise. iS50 ATR Module User Guide Thermo Scientific...
  • Page 9 ( watch video) The tip can be screwed on and off while the pressure arm is raised. Move the arm to the resting position for easy access. The following tip styles are available. Thermo Scientific iS50 ATR Module User Guide...
  • Page 10 • Make sure the sampling plate is installed before you place a sample on the ATR crystal. • Avoid damaging or contaminating the ATR internal optics. Keep samples and other lab materials away from the ATR module when the sampling plate has been removed. iS50 ATR Module User Guide Thermo Scientific...
  • Page 11 The OMNIC window is displayed. For information about the OMNIC window, choose Help > OMNIC Help Topics. 2. Choose the ATR Touch Point button in the software to configure the instrument for ATR sampling. Thermo Scientific iS50 ATR Module User Guide...
  • Page 12 The Touch Point Setup dialog box provides commonly used settings that define how your ATR data will be collected, displayed, measured and reported. Brief descriptions are provided below. iS50 ATR Module User Guide Thermo Scientific...
  • Page 13 Max/Min Shows the default limits of the selected detector’s spectral range. The Max/Min limits can be reduced to eliminate spectral regions that provide no additional information about the sample. Thermo Scientific iS50 ATR Module User Guide...
  • Page 14 “Finding Peaks Above a Specified Height” topic. • Threshold. The Y value above which peaks can be found. • Sensitivity. Determines how readily shoulders on peaks and small peaks in the baseline will be found. iS50 ATR Module User Guide Thermo Scientific...
  • Page 15 • Select an appropriate film or other solid for your sample (we use a yellow sticky note for this experiment). • Make sure the sampling plate, pressure tower and appropriate are installed. • Remove any previous sample from the sampling area and raise the pressure arm. Thermo Scientific iS50 ATR Module User Guide...
  • Page 16 1. Press the ATR Touch Point to select the ATR beam path and load its default workflow. A message may be displayed while the instrument collects scans to determine stability with the new configuration. iS50 ATR Module User Guide Thermo Scientific...
  • Page 17 6. Enter a title for your sample spectrum and choose OK (or press the ATR Touch Point to accept the default title). If the software has no appropriate background spectrum in memory, a message tells you to prepare for the background collection. Thermo Scientific iS50 ATR Module User Guide...
  • Page 18 If your workflow specifies an analysis such as quantify or search, those steps occur automatically. To see the analysis results, choose the “r” button to the left of the title box above the OMNIC spectral window. iS50 ATR Module User Guide Thermo Scientific...
  • Page 19 Raise the pressure arm and move it to the resting position or install the liquid sampling kit. • Make sure the crystal is clean. For cleaning instructions and recommended solvents, see “Cleaning the Crystal.” Thermo Scientific iS50 ATR Module User Guide...
  • Page 20 1. Press the ATR Touch Point to select the ATR beam path and load its default workflow. A message may be displayed while the instrument collects scans to determine stability with the new configuration. iS50 ATR Module User Guide Thermo Scientific...
  • Page 21 5. Enter a title for your sample spectrum and choose OK (or press the ATR Touch Point to accept the default title). If the software has no appropriate background spectrum in memory, a message tells you to prepare for the background collection. Thermo Scientific iS50 ATR Module User Guide...
  • Page 22 If your workflow specifies an analysis such as quantify or search, those steps occur automatically. To see the analysis results, choose the “r” button to the left of the title box above the OMNIC spectral window. iS50 ATR Module User Guide Thermo Scientific...
  • Page 23 Typical ATR Spectra The background spectrum shows the response of the spectrometer and the ATR module without a sample in place. Below is a typical background spectrum collected with the iS50 ATR module. Thermo Scientific iS50 ATR Module User Guide...
  • Page 24 2300 cm and 2000 cm and may mask or interfere with any sample absorptions that occur in that region. See the section titled “Typical ATR Sample Spectrum” “Tips for ATR Sampling” for more information. iS50 ATR Module User Guide Thermo Scientific...

  • Page 25: Maintenance

    ATR Module Sampling Accessories for the ATR Module Optional Thermo Scientific accessories for the iS50 ATR module are highlighted below. To order an accessory, call our local sales or service representative or use the information at the beginning of this document or help file to contact us.
  • Page 26 4. Dampen another clean paper napkin with alcohol and use it to wipe the crystal. 5. If the sample is oily or viscous, place a drop of alcohol on the crystal and wipe it with another clean napkin. 6. Dry the crystal with another clean napkin. iS50 ATR Module User Guide Thermo Scientific...
  • Page 27 NOTICE Prolonged exposure to strong acids or bases may stain the sampling plate. When the sampling plate is clean, install it on the ATR module. See “Removing and Installing the Sampling Plate” for more information. Thermo Scientific iS50 ATR Module User Guide...

  • Page 28: Troubleshooting

    Hardware problems Steps you can take to resolve problems with the ATR module. Applications problems How to fix problems in the collected spectra. Error messages How to deal with error messages you may see. iS50 ATR Module User Guide Thermo Scientific...
  • Page 29 • You changed a component in your spectrometer or the ATR module. • You changed one of the Collect, Bench or Advanced settings in the selected experiment (except Gain, Final Format, Number Of Scans or Correction). Thermo Scientific iS50 ATR Module User Guide...
  • Page 30 • The sample concentration is low or the sample is weakly absorbing. If you are measuring a liquid, concentrate it if possible. If you are measuring a solid, the sample may not be compatible with the diamond ATR technique. iS50 ATR Module User Guide Thermo Scientific...
  • Page 31 • Reseat the beamsplitter either manually or by choosing Collect > Experiment Setup > Diagnostic > Reset iS50 ABX. For information about installing a beamsplitter manually, open Spectrometer Help Topics and find “Installing, Removing and Storing Beamsplitters.” Thermo Scientific iS50 ATR Module User Guide...
  • Page 32 • If the laser is still not visible through the ATR crystal, choose Collect > Advanced Diagnostics and click the Laser icon to check its status. If a laser test has failed, contact our local service representative for technical support. iS50 ATR Module User Guide Thermo Scientific...
  • Page 33 ATR module sample spectrum chemicals described reactive with diamond ATR crystal typical cleaning sampling plate ATR module housing installing ATR module sampling plate removing coadding Thermo Scientific iS50 ATR Module User Guide...
  • Page 34 ATR module, selecting ATR module mid-IR region experiments ATR module, selecting ATR module, default extended range ATR module negative sample peaks ATR module, troubleshooting noisy sample spectrum Far-IR region iS50 ATR Module User Guide Thermo Scientific...
  • Page 35 ATR module ATR module Quantify option scans ATR module, setting ATR module quantitative analysis method averaging ATR module, selecting setting Search option ATR module, setting range Sensitivity option ATR module, extending ATR module, setting Thermo Scientific iS50 ATR Module User Guide...
  • Page 36 ATR module, entering Touch Point ATR module Touch Point options overlapping with Experiment Setup options Touch Point Setup dialog box ATR module settings troubleshooting ATR module iS50 ATR Module User Guide Thermo Scientific...