Thermo Scientific Ion 520 User Manual page 61

Observation
Instrument will not begin a run
Instrument displays one or
more alerts during a run
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
Possible cause
The instrument has
encountered a Deck Scan error
(one or more consumables are
absent or loaded improperly).
The instrument has
encountered an internal error.
The instrument detected one or
more problems during the run.
Appendix A Troubleshooting
Ion Chef ™ System setup and operation
Recommended action
1. Confirm that the touchscreen does
not display any Deck Scan warnings.
If alarms are present, note the
error(s) displayed, replace the missing
consumable as directed, tap No when
prompted then tap Next to cancel the
run. After returning to the home screen,
restart the run.
2. If the error persists, confirm that:
• All buckets are seated correctly in
the rotors of the Recovery and Chip-
Loading Centrifuges.
• All cartridges are loaded correctly
and are level on the instrument deck.
• The barcodes of the Library Sample
Tubes are visible and positioned
correctly.
• All tubes are both present and
uncapped on the Ion S5
Chef Reagents (sample tubes, NaOH
tube, and the empty tube).
3. If the error persists after you check the
consumables on the instrument deck, do
one of the following:
• If you are confident that the
™
Ion Chef Instrument is set up
correctly and you are comfortable
disregarding the warnings, tap YES
following Deck Scan to proceed with
the run.
• If the instrument cannot begin the
run, contact Technical Support for
further assistance.
1. Record the error displayed on the
instrument display, then tap OK.
2. Contact Technical Support to report the
problem and for further assistance.
After the instrument completes the run,
contact Technical Support. If possible, capture
an image of the alert or error message to help
troubleshoot.
IMPORTANT!
The detected problem might
impact the performance of the sequencing
run.
A
™
ExT
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