Table of Contents
Advertisement
6
■
Chip handling guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
■
Start the sequencing run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
■
Maintain the sequencer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
■
Reagent consumables disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Chip handling guidelines
To place a chip in the chip clamp, perform the steps that are described in the following figure:
1
Slide the chip clamp out.
1
Remove the chip currently in the clamp.
2
Place the appropriate loaded chip in the chip clamp with the chip notch in the bottom‑front corner.
3
Note: Do not force the chip into the clamp. If the chip does not fit easily in the clamp, confirm that the notch is
oriented as shown in the drawing.
Slide the metal tab in fully to engage the clamp, then close the instrument door.
4
Start the sequencing run
We recommend that you start a sequencing run as soon as possible after chip loading and instrument
initialization are complete. However, successful sequencing runs can be started up to 24 hours after
instrument initialization.
IMPORTANT!
·
Do not press the power button during a run. Interrupting power to the instrument during a run can
result in sequencing run failure and loss of sample.
·
We recommend that you verify the status of the Planned Run you will use as "Planned" before
inserting the loaded chip. If your Planned Run does not advance to the "Planned" status in Torrent
™
Suite
Software after successful completion of an Ion Chef
advance to "Planned""
Ion 520
™
& Ion 530
™
ExT Kit − Chef User Guide
Start the sequencing run
2
on page
62.
3
™
run, see "Planned Run status does not
4
49
Advertisement
Table of Contents
