Start The Ion Chef; Materials Required; Dilute The Library - Thermo Scientific Ion 520 User Manual

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Materials required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
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Dilute the library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
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Prepare the consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
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Add the diluted libraries to the Ion Chef
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Load the Ion Chef
■
Start the Ion Chef
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Unload the chip for sequencing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
This chapter describes how to perform the following procedures:
• Set up the Ion Chef
of the required reagents and consumables
• Start an Ion Chef
• Unload the chips for sequencing

Materials required

™
• Ion 520
& Ion 530
• Ion 520 ™
Chip or Ion 530
• Library or combined library stock solution
• Molecular-biology grade nuclease-free water
• P200 pipettor and filtered tips
• Waste container

Dilute the library

Sample libraries are quantified at the end of the library preparation workflow and are typically diluted
to a concentration of 100 pM. The Ion Library Equalizer
concentration to 100 pM. Libraries may require further dilution before use in Ion Chef
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™
™
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
™
run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
™
Instrument for use by diluting the libraries, and loading the instrument with all
™
run
™
ExT Kit - Chef (Cat. No. A30670)
™
Chip

Start the Ion Chef

Library Sample Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
™
Kit procedure also normalizes library
Ion 520
™
™
template runs.
™ & Ion 530 ™ ExT Kit − Chef User Guide
run