Thermo Scientific Ion 520 User Manual page 41

b. Transfer the entire volume of the QC sample from Position A of the Ion S5
Reagents cartridge on the instrument deck to a new labeled microcentrifuge tube.
IMPORTANT!
·
Do not remove the Library Sample Tubes from the Ion S5
·
If you unintentionally close the instrument door before you obtain the QC sample, you must
wait until the end of the run before you can collect it. You cannot pause the run or open the
door after it has been closed.
Position A (QC sample)
1
c. If you are performing quality assessment of an enriched sample, transfer the QC sample from
Position E of the Enrichment Cartridge v2 to a new labeled microcentrifuge tube.
d. Analyze the QC sample. For more information, see "Quality control of Ion Sphere
on page
e. Close the instrument door, then tap Continue to complete the run.
13. When the run is complete, unload the Ion Chef
You can collect QC samples from the Reagents and/or Enrichment cartridges if you have not done
so already.
IMPORTANT! Liquid may be present in the chip wells after the Ion Chef
any residual liquid from the wells.
Note: If you cannot sequence a loaded chip immediately, place the chip into a separate chip
storage container and store at 4°C until you are ready to sequence it (up to 6–8 hours maximum).
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
A
00012216
B
C
D
67.
1
™
Instrument and sequence the chip immediately.
Chapter 4 Start the Ion Chef
Start the Ion Chef
™
ExT Chef
™
ExT Chef Reagents cartridge.
™
™
run. Do NOT remove
™ run
4
™
run
Particles"
41