Water Quality Recommendations; Prefilter - Thermo Scientific HERACELL VIOS 160i LK User Manual

Water Quality Recommendations:

For trouble-free operation, fill the water reservoir with sterilized distilled water or water treated to an
equivalent quality level. The acceptable conductivity should be within the range of 1 to 20 S/cm
(resistivity should be within the range of 50 kOhmcm to 1 MOhmcm).
CAUTION Termination of warranty!
Using chlorinated tap water or additives that contain chlorine will void the manufacture warranty.
Similarly, the use of ultrapure water whose conductivity is out of the range of 1 to 20 S/cm and
whose resistivity is out of the range of 50 kOhmcm to 1 MOhmcm will void the manufacture
warranty.
If you should have any questions, please contact Technical Support of Thermo Fisher Scientific.
CAUTION No tap or ultrapure water in humidity reservoir
Sterile distilled water or water treated to an equivalent quality level is the recommendation for use
in the integrated humidity reservoir. The acceptable conductivity should be within the range of 1 to
20 μS/cm (resistivity should be within the range of 50 K-Ohm-cm to 1 M-Ohm-cm). The pH
should be in the range of 7-9. Ultrapure Type 1 water or deionized (DI) water with a resistivity
approaching or equal to 18.2 M-Ohm-cm, contains very few ions and will actively pull ions from
interior components, damaging stainless steel, copper, and glass. If there is only access to DI or
Type 1 water, one option is to add a sterile solution of weak sodium bicarbonate to raise the pH and
add ions (recommend 84 mg/l (1 mmol/l).
CAUTION No chloride containing disinfectants
While stainless steel is resistant to corrosion, it is not corrosion proof. Many chemicals have a
negative effect on stainless steel, especially chlorine and derivatives with oxidizing activity.
The addition of chloride-containing disinfectants or copper sulfate into the water as a constant
disinfectant is not recommended because these can damage the connecting stainless steel/copper
joint of the drain.For cleaning the interior, the recommendation is mild soap solution in water then
rinse to remove residues. Wipe the interior surfaces and parts with a diluted quaternary ammonium
disinfectant. Follow this by wiping with 70% alcohol to remove any remaining traces of the
disinfectant.
Under normal operating conditions and at the usual incubation temperature of 37 °C, a constant
relative humidity of approx 93% is achieved in the workspace.
If condensation occurs on the culture containers due to high relative humidity, the humidity in the
workspace can be set to a lower level. The relative humidity in the workspace is lowered from approx.
93% to approx. 90%. The modification requires an extended adaption phase. To ensure that it
effectively prevents dew formation on culture containers, it must be used as a permanent setting.
Instructions for activating the Low Humidity function are given in section
page 6-33.

Prefilter

Placed on the front section of the water reservoir cover is a pre-filter. The pre-filter consists of a
dual-layer wire mesh with a silicone frame and is autoclavable as well as heat-resistant. The pre-filter
must remain installed in the device during the Steri-run decontamination routine, but needs to be
removed for refilling the water reservoir.
Thermo Scientific
3
Description of the Device
Workspace Atmosphere
"Low Humidity Setting"
HERA 160i LK / 250i LK
CELL VIOS
on
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