Appendix B Supplemental Procedures; Perform A Manual Cleaning Of The Sequencer - Thermo Scientific Ion 520 User Manual

B
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Perform a manual cleaning of the sequencer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

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Perform an instrument reset run with an initialized sequencer that is loaded with an
unused Reagents cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
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Quality control of Ion Sphere
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Maintain the Ion Chef
Perform a manual cleaning of the sequencer
A cleaning protocol is normally performed automatically at the completion of each sequencing run. If a
cleaning is necessary, perform the following procedure.
1. On the home screen, select Settings4Clean Instrument.
The instrument door unlocks allowing access to the consumables.
2. Remove the Ion S5
empty the waste reservoir.
3. Reinstall the empty waste reservoir and a used Ion S5
4. Ensure that the Ion S5
are properly installed.
IMPORTANT! Perform the cleaning with a used reagent cartridge and wash solution bottle
installed. The cleaning procedure pumps cleaning solution into the wash solution bottle and
reagent cartridge making them unsuitable for sequencing.
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
Supplemental procedures
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Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
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System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
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ExT Wash Solution bottle to access the waste reservoir, then remove and
2
1
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ExT Sequencing Reagents cartridge and Ion S5
3
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ExT Wash Solution bottle.
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ExT Wash Solution bottle
65