Guidelines For Using Ion Chef ™ Consumables And Reagents; Prepare The Ion Chef ™ Instrument For Use - Thermo Scientific Ion 520 User Manual

Guidelines for using Ion Chef
To ensure the proper function of the Ion Chef
reagents according to the following guidelines:
• Store all consumables and cartridges under the recommended conditions and in an upright
position.
• Inspect all consumables and cartridges for damage upon arrival and again before use.
• Hold sequencing chips by gently gripping them by their edges. Do not wear gloves when handling
chips.
• When the instrument is not in use, remove all consumables and reagents from the deck and close
the instrument door.
• Except for the new Ion S5
After each run, transfer the empty Ion S5
waste container for the next run.
IMPORTANT! All Ion Chef
• Use only Ion Torrent
reagents and supplies can adversely affect the performance of the Ion Chef
prepared.
• Remove and sequence chips within 1 hour after the Ion Chef
cannot sequence a loaded chip immediately, store it within a chip storage container at 4°C until
you are ready to run it (up to 6–8 hours maximum).
IMPORTANT! If you choose to store a loaded chip, remove the chip from 4°C storage (but keep
it in the chip storage container) at least 20 minutes before running it, allowing the chip to warm to
room temperature.
Prepare the Ion Chef
• Ensure that the Ion Chef
instrument before loading it with consumables.
Note: For more information on the cleaning procedure, see Chapter 7, "Clean the Ion Chef
System".
• Inspect the empty compartments of the Reagents and Solutions stations for condensation.
Condensate can collect in these compartments, depending on temperature and humidity
conditions. Before loading consumables into the instrument, wipe the compartments dry with a
laboratory wipe or absorbent cloth, if needed.
• Thaw the Ion S5
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
™
ExT Tip Cartridge, do not reuse any of the consumables or reagents.
™
Instrument components are single-use only.
™
kits and supplies with the Ion Chef
™
Instrument for use
™
Instrument has been cleaned following the previous run. If not, clean the
™
ExT Chef Reagents cartridge at room temperature for 45 minutes before use.
Guidelines for using Ion Chef
™
consumables and reagents
™
System, handle the associated consumables and
™
ExT Tip Cartridge to the waste tip station for use as the
™
Instrument. The use of third-party
™
System finishes loading them. If you
Chapter 2 Before you begin
™ consumables and reagents
™
Instrument and chips
™
2
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