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Chapter 5 Initialize the sequencer
5
Before you begin
Note:
·
The system uses RFID technology to verify that the proper reagents are loaded in positions 3, 5, and
6. Reagents that exceed their expiration date or usage count generate an error message prompting
the user to replace the reagent before performing the run.
·
RFID regulatory information can be found on the main screen under Options4Regulatory info.
Before you begin
™
The Ion S5
Sequencer, Ion S5
equipped to verify the compatibility of each chip and consumable that is loaded during initialization
and sequencing, and that these components do not exceed their expiration date. To avoid exceptions
during initialization, inspect this information for each consumable before installing onto the instrument.
• Unbox the Ion S5
equilibrate to room temperature.
Do not remove the Ion S5
immediately before loading, so that you can return the unused cartridge to storage if your
sequencing run is delayed.
• Unbox the Ion S5
bag, then swirl at an angle to mix thoroughly.
• Remove the Ion S5
cap from the Ion S5
installing on the instrument.
When a manual cleaning of the sequencer is required
™
The Ion S5
Sequencer, Ion S5
that a cleaning be performed before initialization. Cleaning is normally performed automatically at the
completion of the previous sequencing run. When two sequencing runs are performed on a single
initialization, the post-run cleaning is performed after the second sequencing run. However, if the
"Enable post-run clean" checkbox (see "Start the sequencing run"
allow a second run, and a second run is not performed, the instrument will not allow the subsequent
initialization to proceed until a manual cleaning has been performed. For more information on how to
perform a manual cleaning, see "Perform a manual cleaning of the sequencer"
If the sequencer is initialized and a sequencing run is not started within 24 hours, or a run is not started
or completed due to a power failure or an abort, do not perform a manual cleaning. An instrument reset
run is required before reinitialization. For more information, see "Perform an instrument reset run with an
initialized sequencer that is loaded with an unused Reagents cartridge"
46
™
XL Sequencer, and Ion GeneStudio
™
ExT Sequencing Reagents cartridge 45 minutes before use, then allow it to
™
ExT Sequencing Reagents cartridge from its packaging until
™
ExT Wash Solution bottle. Invert the bottle 5 times within its vacuum-sealed
™
ExT Wash Solution bottle from its vacuum-sealed bag, then remove the red
™
ExT Wash Solution and Ion S5
™
XL Sequencer, and Ion GeneStudio
™
S5 Series Sequencer are
™
Cleaning Solution bottles immediately before
™
S5 Series Sequencer require
on page
49) is deselected to
on page
on page
Ion 520
™
& Ion 530
™
65.
66.
ExT Kit − Chef User Guide
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