Load The Tip Racks And Pcr Plate - Thermo Scientific Ion 520 User Manual

Chapter 4 Start the Ion Chef
4
™
Load the Ion Chef System

Load the tip racks and PCR Plate

1. Tap
(Open Door) in the instrument touchscreen to open the instrument door, then wait for the
latch to open.
2. Lift the instrument door to the top of the travel until the latch mechanism engages.
1
Hold here, then lift
1
3. Load an empty pipette tip rack to the Used (Waste) Pipette Tip Position, then change gloves.
Used Pipette Tip Position
1
IMPORTANT!
· Confirm that the pipette tip rack in the Used (Waste) Pipette Tip Position does not contain any
tips. The instrument will abort the run if tips are present in the Used position.
·
To prevent contamination, change gloves immediately after moving the empty pipette tip rack to
the Used (Waste) Pipette Tip Position.
Note: A small amount of dried residue may be present in the tub of the empty pipette tip rack after
a run. This will not affect the next run.
4. Unwrap a new Ion S5
load it in the New Pipette Tip Position.
Note:
·
Two Ion Chef
Cartridge.
· Keep the cover for later use.
28
™ run
™
ExT Tip Cartridge and remove the cover to expose the pipette tips, then
™
Piercing Tips are pre-loaded into tip positions G7 and H7 on the Ion S5
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
™
ExT Tip