Load The Reagents And Solutions Cartridges - Thermo Scientific Ion 520 User Manual

5. Slide the catch forward to allow the locking bracket to pivot upward. Load the Ion S5
Cartridge into the New Pipette Tip Position, pull the bracket downward, then push the catch
backward to lock the cartridge in place.
New Pipette Tip Position
1
Bracket
2
6. Load a new PCR Plate into the thermal cycler sample block, then insert a new Frame Seal v2
underneath the automated heated cover.
IMPORTANT! When the Frame Seal v2 is positioned correctly, its tabs project upward and
contact the heated cover.
A
B
C
D
E
F
G
H
Thermal cycler sample block
1
Well A1
2

Load the Reagents and Solutions cartridges

IMPORTANT! Thaw the Reagents cartridge at room temperature for 45 minutes before use.
1. Gently tap the Ion S5
bottoms of the tubes.
2. If bubbles are present below the surface of the liquid, repeat step 1.
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
1 2 3 4 5 6 7 8 9 10 11 12
™
ExT Chef Reagents cartridge on the bench to force the reagents to the
Chapter 4 Start the Ion Chef
Load the Ion Chef
Catch
3
™
New Ion S5 ExT Tip Cartridge
4
Cover
3
Keyed corner
4
™ run
4
™
System
™
ExT Tip
29