Chapter 1 Product Information; Product Description; Library Compatibility; Ion Genestudio - Thermo Scientific Ion 520 User Manual

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Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

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Kit contents and storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
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Related products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
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Required materials not supplied . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
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About the Ion Chef
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Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
IMPORTANT! Before using this product, read and understand the information in the "Safety" appendix
in this document.
Product description
™
The Ion 520 & Ion 530
are required to perform the following steps in the sequencing workflow.
• Prepare enriched, template-positive Ion Sphere
Chip or Ion 530
• Sequence the loaded chips on an Ion S5
S5 Series Sequencer.
™
The Ion Chef Instrument uses automated isothermal amplification technology with the Ion 520
™
Ion 530 ExT Kit – Chef to prepare 400 to 600‑base‑read templates with greatly reduced run times
compared to emulsion PCR-based templating. Each kit contains all the supplies that are required
to load and sequence 4 chips. The kit supports 4 single-chip Ion Chef
initializations (1 sequencing run/sequencer initialization).

Library compatibility

™
The Ion 520 & Ion 530
type prepared using any available Ion Torrent

Ion GeneStudio

™
In this document, Ion GeneStudio
generically to the following systems, unless otherwise specified.
• Ion GeneStudio
• Ion GeneStudio
• Ion GeneStudio
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™
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
™
ExT Kit – Chef (Cat. No. A30670) contains all the reagents and materials that
™
Chip using the Ion Chef
™
ExT Kit – Chef can be used with up to 400 to 600-base-read libraries of any
S5 Series instrument reference
™
S5 Series Sequencer or Ion GeneStudio
™
S5 System (Cat. No. A38194)
™
S5 Plus System (Cat. No. A38195)
™
S5 Prime System (Cat. No. A38196)
Product information
™
Particles (ISPs) and load them onto an Ion 520
™
Instrument.
™ ™
Sequencer, Ion S5 XL Sequencer, or Ion GeneStudio
™
-branded library kit.
Ion 520
™
runs and 4 sequencer
™
S5 Series System refers
™ & Ion 530 ™ ExT Kit − Chef User Guide
™
™
™
&