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Chapter 4 Start the Ion Chef
4
™
Load the Ion Chef
System
3. Load the cartridge into the Reagents station so that it snaps into place and is level on the deck.
IMPORTANT! Do not force the Ion Chef
location on the deck and in one orientation. If a cartridge does not fit, confirm that you are loading
the correct cartridge in the correct orientation.
Reagents station (4°C)
1
4. Uncap, then load the Ion Chef
Position A on the Reagents cartridge.
Position A (Library Sample Tube)
1
Position B (2 M NaOH)
2
IMPORTANT! Orient the Ion Chef
oriented to the right.
5. Uncap the tube of 2 M NaOH in Position B and the tubes in Positions C and D, each containing an
™
Ion S5
ExT Pellet.
IMPORTANT! When the Reagents cartridge is loaded:
·
Press down on the Library Sample Tubes to ensure that it is firmly seated in the cartridge.
·
Ensure that all four tubes are uncapped.
6. Gently tap the Ion S5
of the tubes.
30
™
run
1
2
3
4
5
6
7
8
9
10
11 12
A
B
C
D
E
F
G
H
™
Library Sample Tube containing 50 µL of diluted library into
A
1
00012216
B
2
C
3
D
4
™
™
Chef Solutions cartridge on the bench to force the reagents to the bottoms
™
cartridges into place. Each cartridge fits only one
™
Ion S5
2
Position C (Ion S5
3
Position D (Ion S5
4
Library Sample Tube so that the barcode is visible and
Ion 520
ExT Chef Reagents cartridge
™
ExT Pellet)
™
ExT Pellet)
™
& Ion 530
™
ExT Kit − Chef User Guide
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