Thermo Scientific Ion 520 User Manual page 40

Chapter 4 Start the Ion Chef
4
™
Start the Ion Chef run
Note: Select Pause if you are uncertain of library quality and want to evaluate templating
efficiency before the chip is loaded. If you do not pause the run, you can collect QC samples
after the run. Save the samples until sequence analysis is complete to have them available for
troubleshooting.
10. On the Run Options screen, tap Start run to start the run.
Note: If you need to stop the run for any reason, tap Cancel, then tap Yes to confirm the
cancellation.
If the Ion Chef
error on the instrument touchscreen. If a run fails, perform the following tasks.
a. Remove the consumables from the deck, then clean the instrument. If possible, retain the
consumables for troubleshooting.
b. Reset, then attempt to start the run again. If the run fails again, contact Technical Support to
troubleshoot the problem. Record the error message for reference.
11. Initialize the sequencer at least 50 minutes before the Ion Chef
Chapter 5, "Initialize the sequencer".
12. If you chose to pause the run to analyze the templating efficiency, remove the sample for testing
when prompted to do so by the Ion Chef
a. When prompted to remove the QC sample, open the instrument door.
40
™ run
™
Instrument encounters a problem during the run, it aborts the run and displays the
™
System finishes chip loading. See
™
Instrument (approximately 5.75 hours into the run).
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide