Thermo Scientific Ion 520 User Manual page 50

Chapter 6 Start the sequencing run
6
Start the sequencing run
1. After completion of initialization, tap Run in the instrument touchscreen. The door and chip clamp
unlock.
2. Remove the used sequencing chip, then fasten a chip that is loaded with template-positive Ion
™
Sphere Particles in the chip clamp.
3. Push the chip clamp all the way in to engage, close the instrument door, then tap Next.
Note: Do not remove the chip from the chip clamp until completion of the run. Removing and
reinserting the chip risks introducing air bubbles in the chip.
4. Confirm that the correct Planned Run has auto-populated, then tap Review.
Note: Ensure the Enable post-run clean checkbox is selected so that the post-run cleaning is
performed automatically.
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Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide