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Load the Chip-loading centrifuge
1. Load the chip that you will use for templating and
sequencing into a centrifuge bucket, then attach a
Chip Adapter to the assembly.
a. Place the chip in the chip-loading bucket, then
align the wells of the Chip Adapter to the wells of
the chip, orienting the adapter onto the chip so
that the chip barcode is visible.
b. Place the adapter onto the chip, then insert the
stationary tabs at the reservoir end of the adapter
into the slots of the bucket.
c. Gently squeeze the flexible tabs at the other end
of the adapter into the bucket slots until the
adapter locks into place.
d. Confirm that the tabs at all four corners of the
adapter are fitted into the slots in the centrifuge
bucket. Loading can fail if the adapter is not
attached securely.
Note: If desired, you can label the tops of chips to
distinguish them. Do not obstruct or overwrite the chip
barcode with the label.
2. Load the adapter/chip/bucket assembly into Position 1 of the Chip-loading centrifuge.
Chip-loading centrifuge
1
Position 1
2
Position 2 marker holes
3
Note: Position 1 of the Chip-loading centrifuge is the position 90° clockwise from the single hole
in the rotor bucket cover at rest. The chip loaded in Position 1 is loaded with ISPs prepared from
the DNA library in the Library Sample Tube loaded in Position A of the Reagents cartridge.
Ion 520
™
& Ion 530
™
ExT Kit − Chef User Guide
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A
B
C
D
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F
G
H
Chapter 4 Start the Ion Chef
Load the Ion Chef
1
2
3
1
Chip Adapter
2
™
Ion Chip
3
Bucket
4
Reservoir end of Chip Adapter
5
Ports (align with chip)
6
Tabs
7
Keyed corner (align with bucket)
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Slots
Position 2
4
Position 1 marker hole
5
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run
4
™
System
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