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4.3.3 Separation using an
AutoSampler Module
4.3.4 Using the Queue Tab
4. 10 mL of weak solvent is aspirated through the probe to
wash away any remaining strong solvent from the probe so
it cannot interfere with future separations.
Verify that the wash station contains fluid. If not, go to
T
|A
OOLS
UTOMATION
W
button. Once the wash station is primed, select the S
ASH
W
button. Ensure that the wash fluid supply container has
ASH
sufficient clean wash fluid for the planned separations.
In addition, if the separations will use a solvent bracketed
injection ensure there is sufficient bracket solvent for the
planned separations. This solvent is placed into an 18 mm test
tube located to the left of the wash station. The system uses
~100µl per injection. The probe can only aspirate fluid down
to the level of the top of the tube holder so the tube must be
filled to a level above that point.
To begin a separation sequence with the AutoSampler Module,
you can create a sample queue entry or simply press the P
button to start with a single sample. Accessing the R
result in a screen and operation much like the standard
ACCQPrep (without an installed AutoSampler Module). Pressing
P
will display the R
LAY
you can perform a manual injection by removing the Luer fitting
from the inject loop, or you can perform multiple injections on a
single sample from the AutoSampler Module. A queue will be
created automatically. Selecting the Q
set up a separation sequence for multiple samples.
Note
If you are performing a manual injection, the Luer fitting or a
needle port must be reinstalled on the injection valve.
This section outlines the operating procedures for the
AutoSampler Module.
1. Install a sample vial rack before opening the Q
This will allow the software to limit sample programming
to the positions available for sample vials.
2. Open the Q
UEUE
The following is an explanation of the available columns on this
screen:
= – Allows you to start the separation from the Queue tab, to
remove a single row of an existing queue, clear all rows of the
queue, or enable or disable a scouting pause. The scouting pause
halts the system after the first separation is completed for the
sample. This allows you to adjust the separation parameters
based on the results of the initial separation. This is especially
useful to allow adjustment of the quantity of sample injected for
each separation to optimize the loading on the column. After the
initial pause, you can modify the method as needed and disable
the pause for the remaining injections, or leave it enabled to
allow a second condition scouting separation.
Section 4 Operation
M
C
and select the S
ANUAL
ONTROL
R
screen. On this screen,
UN
EQUIREMENTS
tab will allow you to
UEUE
tab.
ACCQPrep HP125
TART
TOP
LAY
tab will
UN
tab.
UEUE
4-7
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Table of Contents
Troubleshooting
