Teledyne ACCQPrep HP125 Installation And Operation Manual page 50

ACCQPrep HP125
Section 4 Operation
Figure 4-2 Selecting the sample volume and number of
injections
4-4
PeakTrak will divide the total volume by the number of injec-
tions to determine the injection volume for each separation. If
this volume exceeds 50% of the loop volume, a warning message
will appear recommending a change in the parameters. This
message can be ignored at your discretion. PeakTrak will
perform exactly as programmed. It will also compensate for the
volume of tubing used for the supplied sample probe. If the
sample probe is ever lost or damaged, it should be replaced by
the AutoInjector Needle Assembly (PN 60-5234-657).
During the programmed separation sequence, any changes made
to the method will be included on any of the remaining injections.
This includes automatic changes such as using the peak hold
feature or automatic run length extension. For the most reliable
operation, it may be best to disable these automatic features.
Mid-separation flow rate changes will occur at the beginning of
the next separation. Please be aware that changes of flow rate
will not automatically affect the overall run length.
5. After the last injection is performed, a sample probe wash
screen is shown prompting you to wash the probe with a
strong solvent followed by a weak solvent. Perform these
duties as requested.
Note
A weak solvent is used following the usage of the strong sol-
vent to inhibit poor separation due to excessive strong solvent
in the probe tubing. It is important to perform Step 5 to ensure
there is no carryover into the next separation.