Ionization Settings - Teledyne ACCQPrep HP125 Installation And Operation Manual

ACCQPrep HP125
Section 5 PeakTrak

5.2.13 Ionization Settings

5-22
To prime the pump, open the priming valve on the fluid
interface counterclockwise. Use the 5 mL Luer lock
syringe provided with the accessory kit to draw liquid
into the pump. Repeat this procedure once more to
completely fill or purge the line from the carrier solvent
reservoir to the priming valve. Make sure the priming
valve is tightly shut after priming the system.
• Stop Carrier Pump- Stops the carrier pump. This
changes to read START CARRIER PUMP after the
pump is stopped.
• Operate Splitter Valve- Runs the splitter valve to verify
operation.
• Fluid Interface Pressure - The carrier solvent pressure
is displayed in a ribbon gauge. If the pressure
approaches the level of the red line, the system is
clogged and should be cleaned to allow good response.
• Close - Closes the window and stops the carrier pump if
it is running.
The MS->I S
ONIZATION
adjust various settings to improve the ionization of a particular
compound by reducing fragmentation or adduct formation.
• Mass Spectrum Graph - this displays the mass
spectrum. There are two mass spectra displayed. Blue
displays the maximum of the current injection. Red
displays the maximum of the previous injection for
comparison. When a new injection is performed, the blue
spectrum replaces the red spectrum and a new blue
spectrum is started. To inject a sample, move the PurIon
injection valve to the "Inject Sample" position.
Inject the sample (20 uL) and move the valve to the
"Scan Mass" position.
Note
Only use 22 gauge square tip needles (PN 29-9001-911) to
avoid scratching the internal surfaces of the injection valve. Fil-
ter the sample with a 0.45 µ filter to avoid clogging the PurIon
tubing and probe capillary. Sample concentration should be
less than 20 µg/mL.
• Polarity - switches the mass spectrometer between
positive and negative polarity.
Note
The PurIon S and L systems capture both polarities. The setting
changes which polarity is displayed.
• Format Graph- Set the displayed range of the mass
spectrum. The PurIon system still collects the entire
menu command allows a user to
ETTINGS