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3. Pump 100 ml of solvent through the system at 100 mL/min.
4. If an alternative solvent was used, return the solvent inlet
line to the appropriate solvent container.
5. Select the Tools>Auto Prime menu command to prime the
system.
6. Replace the bypass tube with a column and start a run.
The Companion XL will attempt to calibrate the flow cell.
If the calibration is successful, the system is ready for use.
If the alert message persists, refer to the alternative flow
cell cleaning solvents and techniques.
The solvent strength and high flow rate will typically clear the
obstruction and return the flow cell to optimum performance.
9.4.3 Alternative Cleaning Techniques
If the thorough flow cell cleaning instructions do not clear the
obstruction, the following alternative techniques should be
considered.
• Pump water/DMF/methanol (50/25/25) for 3–4 minutes
followed by pumping straight methanol (or ethanol) for
2–3 minutes.
• Pump 5–10 mL DMSO followed by methanol, ethyl
acetate, then hexane.
• After the lines have been filled with solvent, reduce the
flow rate or pause up to 18 hours. Doing so will allow the
solvent to remain longer to dissolve the obstruction.
Section 9 Maintenance
9-5
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