Manual Control Of The Accqprep - Teledyne ACCQPrep HP125 Installation And Operation Manual

5.3.8 Manual Control of the
ACCQPrep
Pumping Solvents
Raw Lamp Energy
Manual Control can assist with method development, mainte-
nance of the system plumbing, and system troubleshooting. To
manually control the ACCQPrep system, first open the MANUAL
CONTROL window by clicking the T
menu command.
To pump either solvent, select the solvent from the Solvent A or
Solvent B lists. Then, click the P
pump the selected solvent. To pump a mixture or Solvent A and
B, adjust the Percentage Solvent B setting and then click the
Pump% B button. When finished, click the S
You can control the flow rate by adjusting the Flow Rate mL/min
setting.
By default, the system pumps the solvent directly to the waste
port., you can also pump the solvent into a collection tube. To do
so, select the Pump Into Tube # option and then select which
tube ("next" or specific tube number).
The left side of the Manual Control window shows a Raw Lamp
Energy gauge. This provides an indication of the UV light mea-
sured by the optical detection system at 254 nm. High lamp
energy (green) means that the flow cell easily passes through a
sufficient UV light source. Lower lamp energy (yellow or red)
means that either the light source is weak or that the flow cell is
obstructed.
• Green – lamp energy is sufficient to detect peaks up to
2.4 Absorbance Units.
• Red – lamp energy is obstructed to a degree that the
system might not reliably detect peaks. If you attempt to
operate the system, peak collection will be forced to
collect all. This prevents diverting desired compounds to
waste.
Depending on what you are doing with the M
functions, low lamp energy could be a normal indication. For
example, a UV-absorbing compound could be present in the flow
cell as you are pumping solvent. Or, the selected solvent absorbs
UV light at 254 nm. Abnormal indications could be a flow cell
blocked by a compound that has precipitated or there is a film
built up on the flow cell.
ACCQPrep HP125
Section 5 PeakTrak
> M C
OOL ANUAL ONTROL
A or P B buttons to
UMP UMP
button.
TOP
C
ANUAL ONTROL
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