Operation Troubleshooting - Teledyne ACCQPrep HP125 Installation And Operation Manual

4.4 Operation
Troubleshooting
When the system moves from the ACCQPrep fraction racks to
the AutoSampler fraction racks, the fluid uses an alternative
path. This leaves a portion of tubing with fluid remaining for the
fraction collector previously used. To prevent loss of any com-
pound, the software monitors for a portion of the separation with
no peaks while using the last available row of the fraction col-
lection rack on that module. If a portion without a peak to collect
is identified, the system moves to the next rack on the next
module (the AutoSampler or ACCQPrep). This may leave a few
tubes unused in the previous rack. If the system is mid peak or
the separation is using collect all, the fluid remaining in the
tubing is placed into one of the tubes in the last row of the rack
before the move to collect any undetected compound and then the
system advances to the next rack.
Injection Valve Leak- refer to on rotor replacement if
needed – (See Section 6.5.1for more information)
• Failure to filter the sample can allow particles to scratch
the valve sealing surface. Filter the sample with a 20
µm or finer filter.
• Dissolve sample in sufficient solvent so that evaporation
of solvent doesn't cause formation of crystals
• Manual injection using a hypodermic needle with
pointed tip can scratch the internal sealing surface
resulting in leaks. When using a needle injection port
only use a 22 gauge blunt tip needle. When using a luer
injection port, only use a luer syringe tip.
• If performing manual injection, ensure that a check
valve is mounted downstream of the injection valve to
prevent solvent from dripping when the syringe is
removed
• If inject pump is installed and the waste connected to
other waste lines with a tee, ensure a check valve is
installed in the injection pump waste line.
• Formic acid modifiers can result in reduced injection
valve seal lifetime. If possible, consider other modifies
such as TFA.
• 100% water as a chromatographic solvent can also
reduce seal lifetime.
Un expected retention time or Variable retention times
for repeated injections –
• Temperature variation of only a few degrees can cause a
visible retention time shift.
• Variable flow rates of the A or B solvent:
· Check valves may require cleaning or replacement
due to contamination from solvents or pump seal
wear particles.
ACCQPrep HP125
Section 4 Operation
4-13