Creating Focused Gradients - Teledyne ACCQPrep HP125 Installation And Operation Manual

ACCQPrep HP125
Section 3 Configuration
Figure 3-3 Define Methods screen
3.4.4 Creating Focused
Gradients
3-6
3. Press N .
EW
4. Enter in the name you have chosen.
5. Once named, press E
6. The newly created method uses the default method
parameters. To modify the new method:
a. Select E .
DIT
b. Make changes as desired in the method editing screen.
c. Exit when completed.
Focused gradients are a convenient way of minimizing method
development. Typically, a universal gradient separation can be
performed on an analytical HPLC system. Based on the retention
time of the analytical separation, a method with an ideal narrow
range gradient (such as 20-30%B) can be selected for optimal
separation of a target peak.
When creating a set of focused methods, keep in mind that the
methods will be displayed in the drop down in the order created.
For example, if you want methods for "10-30%B" and "20-40%B",
etc. you may want to create them in sequential order. To create
other methods see Section 3.4.3.
,
NTER