Waters Alliance e2695 Manuals

Manuals and User Guides for Waters Alliance e2695. We have 2 Waters Alliance e2695 manuals available for free PDF download: Operator's Manual

Waters Alliance e2695 Operator's Manual (306 pages)

Separation module

Brand: Waters | Category: Control Unit | Size: 3.04 MB

General Information

3
1 Getting Started with the Alliance E2695 Separations Module

21
- Separations Module Overview
  
  21
  - HPLC System Configurations
    22
  - Solvent Bottle Tray Kits
    23
  - Control of Chromatographic Functions
    25
  - Spill Protection
    26
  - Record-Keeping Functions
    26
  - Power Supply
    27
- Solvent Management System Overview
  
  27
  - Solvent Blending
    28
  - Prime/Vent Valve
    28
  - Plunger Seal-Wash System
    28
  - Loss of Prime Protection
    29
  - Preferred Plunger Stroke Volume
    29
  - In-Line Vacuum Degasser
    29
- Sample Management System Overview
  
  31
  - Flow Path
    31
  - Normal Flow
    32
  - Injection
    32
  - Carousel Configuration
    32
  - Vial Presence
    33
  - Syringe Assembly
    33
- Operating Configurations
  
  34
  - System Controller Mode
    35
  - No Interaction Mode
    37
  - Remote Control Mode
    37
- Options and Accessories
  
  39
  - Column Heater
    40
  - Column Heater/Cooler
    40
  - Sample Heater/Cooler
    40
  - Syringes
    40
  - Sample Loops
    40
  - Column Selection Valve
    41
2 Install the E2695 Separations Module

45
- Installation Overview
  
  45
- Site Requirements
  
  46
- Firmware and Software Requirements
  
  47
- Unpack
  
  48
- Connect to the Electrical Power Supply
  
  48
- Connect Tubing and Attachments
  
  50
- Signal Connections
  
  69
  - I/O Signal Connections
    71
  - Digital Signal Connections
    75
  - Ethernet Connections
    77
- Add Hardware Options
  
  78
3 Prepare the E2695 Separations Module for Operation

83
- Power-On the Separations Module
  
  83
  - Startup Diagnostic Tests
    83
  - The Main Screen
    84
- Operating Overview
  
  84
  - Screen Display Overview
    85
  - Use the Keypad
    86
  - Enter Values in Parameter Fields
    88
- Configure the Separations Module
  
  91
  - Set Configuration Parameters
    91
  - Configure the Operating Mode
    98
- Prepare the Solvent Management System
  
  103
  - Prepare Solvent Reservoirs
    104
  - Degassing Solvents
    105
  - Prime the Plunger Seal-Wash Pump
    106
  - Prime the Solvent Management System
    107
- Prepare the Sample Management System for Operation
  
  107
  - Purge the System
    108
  - Prime the Needle-Wash Pump
    109
  - Adjust the Seal Pack
    110
  - Load Carousels
    111
- Procedures for Preparing the Separations Module for Operation
  
  112
- Power-Off the Separations Module
  
  114
  - Flush the Flow Path
    115
  - Power-Off the Separations Module
    115
4 Front Panel Control

117
- Routine Startup
  
  117
  - Reinitialize the System
    118
- Load Sample Vials
  
  118
  - Remove the Carousel
    118
  - Load Vials
    118
  - Load the Carousel
    119
  - Add New Sample Vials During a Run
    119
- Monitor Your HPLC System
  
  119
- Perform Direct Functions
  
  123
  - Dry Prime the System
    126
  - Run System Prep
    128
  - Wet Prime the System
    129
  - Purge the 2410 and 410 Reference Cell
    132
  - Equilibrate the System
    132
  - Condition the Column
    133
  - Inject Samples
    133
5 Automatic Runs

135
- System Controller or no Interaction Mode
  135
- Controlled by Empower Software
  136
- Controlled by Masslynx Software
  136
- Perform Automatic Runs in a Standalone Mode
  
  136
  - Run a Sample Set
    137
  - View Sample Sets
    139
  - Perform a Run from a Sample Template
    140
  - Modify a Sample Set During a Run
    141
  - Stop a Run
    142
- Perform Automatic Runs under Empower Software Control
  
  142
- Perform Automatic Runs under Masslynx Control
  
  144
  - Perform an Automatic Run Using Masslynx Software
    144
6 Create Methods, Sample Sets, and Sample Templates

145
- Separation Methods
  145
- Sample Sets
  146
- Sample Templates
  146
- Create and Edit Separation Methods
  
  146
  - Create a Separation Method
    148
  - Edit a Separation Method
    149
  - Copy and Edit a Separation Method
    149
  - Lock and Unlock a Separation Method
    150
- Set Separation Method Parameters
  
  150
  - Set Mobile Phase Screen Parameter Values
    151
  - Set Sample Parameter Values
    157
  - Set Autosampler Parameter Values
    159
  - Set Column Parameter Values
    161
  - Set I/O Parameter Values
    162
  - Set Detector Parameters
    166
- Create and Edit a Sample Set
  
  174
  - Link Rows in a Sample Set
    179
- Create a Sample Template
  
  180
7 Maintenance

181
- Maintenance Considerations
  
  181
  - Safety and Handling
    181
  - Proper Operating Procedures
    182
  - Reinitialize the System
    182
  - Spare Parts
    182
- Maintain the Solvent Management System
  
  183
  - Overview
    183
  - Remove the Head, Seal-Wash Assembly, and Plunger
    185
  - Notes on Replacing the Plunger Seals
    187
  - Replace the Seal-Wash Assembly Seals
    190
  - Clean and Replace the Plunger
    192
  - Replace an Inlet Check Valve Cartridge
    194
  - Replace the In-Line Filter
    196
- Maintain the Sample Management System
  
  197
- Safety and Handling
  
  219
8 Diagnostic Testing and Troubleshooting

219
- Proper Operation
  219
- Use the Error Log
  
  220
- Perform Main Diagnostic Tests
  
  221
  - Exit the Diagnostics Screens
    221
  - Prime the Plunger Seal-Wash Pump
    223
  - Perform the Compression Check
    224
  - Prime the Needle-Wash Pump
    225
  - Adjust the Seals
    226
  - Service Utilities Diagnostic Tests
    228
- Perform Other Diagnostic Tests and Functions
  
  228
  - Perform the Motors and Valves Diagnostic Test
    230
  - Sensors Diagnostic Function
    233
  - Static Leak Test
    234
  - Valve Leak Test
    236
  - Head Removal and Replacement Function
    237
  - Inputs and Outputs Test
    237
  - Keypad Test
    239
  - Display Test
    239
  - Carousel Test
    239
  - Sample Heater/Cooler Test
    239
  - Defrost Sample Heater/Cooler Function
    240
  - Column Heater or Column Heater/Cooler Test
    241
  - Rebuild Injector Valves Function
    242
  - Create a GPV Test Method
    242
  - Verify the Firmware Checksum
    243
- Troubleshoot
  
  243
A Safety Advisories

261
- Warning Symbols
  
  262
  - Specific Warnings
    263
- Caution Advisory
  
  265
- Warnings that Apply to All Waters Instruments and Devices
  
  266
- Warnings that Address the Replacing of Fuses
  
  271
- Electrical and Handling Symbols
  
  273
  - Electrical Symbols
    273
  - Handling Symbols
    274
B Specifications

276
- Physical Specifications
  
  276
- Environmental Specifications
  
  276
- Electrical Specifications
  
  276
- Solvent Management Specifications
  
  279
- Sample Management Specifications
  
  281
- Instrument Control and Communication Specifications
  
  282
C Spare Parts

283
- Solvent Management System Spare Parts
  
  284
- Sample Management System Spare Parts
  
  285
- Separations Module Spare Parts
  
  286
- Vials and Low Volume Inserts
  
  286
D Solvent Considerations

291
- Introduction
  
  291
  - Clean Solvents
    291
  - Solvent Quality
    292
  - Solvent Reservoirs
    292
  - Preparation Checklist
    292
  - Water
    293
  - Buffers
    293
  - Tetrahydrofuran (THF)
    293
  - GPC Solvents and Seal Choices
    293
- Solvent Compatibility
  
  294
  - Solvents to Avoid
    295
  - Solvents to Use
    295
- Solvent Miscibility
  
  297
  - How to Use Miscibility Numbers (M-Numbers)
    299
- Buffered Solvents
  
  300
- Head Height
  
  300
- Solvent Viscosity
  
  300
- Mobile Phase Solvent Degassing
  
  301
  - Gas Solubility
    301
  - Vacuum Degassing
    302
- Wavelength Selection
  
  302
  - UV Cutoffs for Common Solvents
    303
  - Mixed Mobile Phases
    304
  - Refractive Indices of Common Solvents
    305

Waters Alliance e2695 Operator's Manual (234 pages)

Separations Module

Brand: Waters | Category: Control Unit | Size: 3.2 MB

Trademarks
3
Customer Comments
4
Safety Considerations
4
1 Getting Started with the Alliance E2695 Separations Module

19
- Separations Module Overview
  19
  - HPLC System Configurations
    20
  - Solvent Bottle Tray Kits
    20
  - Control of Chromatographic Functions
    22
  - Spill Protection
    22
  - Record-Keeping Functions
    23
  - Power Supply
    23
- Solvent Management System Overview
  23
  - Solvent Blending
    24
  - Prime/Vent Valve
    24
  - Plunger Seal Wash System
    24
  - Loss of Prime Protection
    24
  - Preferred Plunger Stroke Volume
    25
  - In-Line Vacuum Degasser
    25
- Sample Management System Overview
  26
  - Flow Path
    26
  - Normal Flow
    27
  - Injection
    27
  - Carousel Configuration
    27
  - Vial Presence
    28
  - Syringe Assembly
    28
- Operating Configurations
  29
  - System Controller Mode
    29
  - No Interaction Mode
    30
  - Remote Control Mode
    31
- Options and Accessories
  33
  - Column Heater
    33
  - Column Heater/Cooler
    33
  - Column Pre-Heater
    33
  - Sample Heater/Cooler
    33
  - Syringes
    33
  - Sample Loops
    34
  - Column Selection Valve
    34
2 Install the E2695 Separations Module

37
- Installation Overview
  37
- Site Requirements
  38
- Firmware and Software Requirements
  39
- Unpack the Instrument
  39
- External Connections
  40
3 Prepare the E2695 Separations Module for Operation

41
4 Front Panel Control

67
- Routine Start-Up
  67
  - Reinitialize the System
    67
- Load Sample Vials
  67
  - Remove the Carousel
    68
  - Load Vials
    68
  - Load the Carousel
    68
  - Add New Sample Vials During a Run
    68
- Monitor Your HPLC System
  69
- Perform Direct Functions
  72
  - Dry Prime the System
    74
  - Run System Prep
    75
  - Wet Prime the System
    76
  - Purge the 2410 and 410 Reference Cell
    78
  - Equilibrate the System
    78
  - Condition the Column
    78
  - Inject Samples
    79
5 Automatic Runs

81
6 Create Methods, Sample Sets, and Sample Templates

89
- Separation Methods
  89
- Sample Sets
  89
- Sample Templates
  90
- Create and Edit Separation Methods
  90
- Set Separation Method Parameters
  94
  - Set Mobile Phase Screen Parameter Values
    94
  - Set Sample Parameter Values
    100
  - Set Autosampler Parameter Values
    101
  - Set Column Parameter Values
    103
  - Set I/O Parameter Values
    104
  - Set Detector Parameters
    107
- Create and Edit a Sample Set
  113
  - Link Rows in a Sample Set
    116
- Create a Sample Template
  117
7 Maintenance Procedures

119
- Maintenance Considerations
  119
  - Proper Operating Procedures
    119
  - Reinitialize the System
    119
- Spare Parts
  119
- Troubleshooting with Connections INSIGHT
  119
- Safety and Handling
  120
- Maintain the Solvent Management System
  121
  - Overview
    121
  - Remove the Head, Seal Wash Assembly, and Plunger
    123
  - Notes on Replacing the Plunger Seals
    124
  - Replace the Seal Wash Assembly Seals
    126
  - Clean and Replace the Plunger
    128
  - Replace an Inlet Check Valve Cartridge
    129
  - Replace the In-Line Filter
    131
- Maintain the Sample Management System
  133
8 Diagnostic Testing and Troubleshooting

149
- Proper Operation
  149
- Safety and Handling
  149
- Use the Error Log
  150
- Perform Main Diagnostic Tests
  150
  - Exit the Diagnostics Screens
    151
  - Prime the Plunger Seal Wash Pump
    152
  - Perform the Compression Check
    152
  - Prime the Needle Wash Pump
    154
  - Adjust the Seals
    154
  - Service Utilities Diagnostic Tests
    156
- Perform Other Diagnostic Tests and Functions
  156
  - Perform the Motors and Valves Diagnostic Test
    157
  - Sensors Diagnostic Function
    159
  - Static Leak Test
    161
  - Valve Leak Test
    162
  - Head Removal and Replacement Function
    163
  - Inputs and Outputs Test
    164
  - Keypad Test
    165
  - Display Test
    165
  - Carousel Test
    165
  - Sample Heater/Cooler Test
    166
  - Defrost Sample Heater/Cooler Function
    166
  - Column Heater or Column Heater/Cooler Test
    167
  - Rebuild Injector Valves Function
    168
  - Create a GPV Test Method
    168
  - Verify the Firmware Checksum
    168
- Troubleshoot
  169
A Safety Advisories

181
- Warning Symbols
  181
  - Specific Warnings
    182
- Notices
  183
- Bottles Prohibited Symbol
  183
- Required Protection
  184
- Warnings that Apply to All Waters Instruments and Devices
  184
- Warnings that Address the Replacing of Fuses
  187
- Electrical and Handling Symbols
  189
  - Electrical Symbols
    189
  - Handling Symbols
    190
B External Connections

191
- Connect to the Electrical Power Supply
  191
- Connect Tubing and Attachments
  193
- Signal Connections
  207
  - I/O Signal Connections
    208
  - Digital Signal Connections
    211
- Add Hardware Options
  213
C Specifications

217
- Physical Specifications
  217
- Environmental Specifications
  218
- Electrical Specifications
  218
- Solvent Management Specifications
  220
- Sample Management Specifications
  221
D Solvent Considerations

223
- Introduction
  223
  - Clean Solvents
    223
  - Solvent Quality
    223
  - Solvent Reservoirs
    223
  - Preparation Checklist
    224
  - Water
    224
  - Buffers
    224
  - Tetrahydrofuran (THF)
    224
  - GPC Solvents and Seal Choices
    224
- Solvent Compatibility
  225
  - Solvents to Avoid
    225
  - Solvents to Use
    226
- Solvent Miscibility
  228
  - How to Use Miscibility Numbers (M-Numbers)
    229
- Buffered Solvents
  230
- Head Height
  230
- Solvent Viscosity
  230
- Mobile Phase Solvent Degassing
  230
  - Gas Solubility
    231
  - Vacuum Degassing
    231
- Wavelength Selection
  232
  - UV Cut-Offs for Common Solvents
    232
  - Mixed Mobile Phases
    233
  - Refractive Indices of Common Solvents
    234

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Waters Alliance e2695 Manuals

Waters Alliance e2695 Operator's Manual (306 pages)

Table of Contents

General Information

Trademarks

Customer Comments

Contacting Waters

Safety Considerations

Operating this Instrument

EC Authorized Representative

1 Getting Started with the Alliance E2695 Separations Module

Separations Module Overview

Solvent Management System Overview

Sample Management System Overview

Operating Configurations

Options and Accessories

2 Install the E2695 Separations Module

Installation Overview

Site Requirements

Firmware and Software Requirements

Unpack

Connect to the Electrical Power Supply

Connect Tubing and Attachments

Signal Connections

Add Hardware Options

3 Prepare the E2695 Separations Module for Operation

Power-On the Separations Module

Operating Overview

Configure the Separations Module

Prepare the Solvent Management System

Prepare the Sample Management System for Operation

Procedures for Preparing the Separations Module for Operation

Power-Off the Separations Module

4 Front Panel Control

Routine Startup

Load Sample Vials

Monitor Your HPLC System

Perform Direct Functions

5 Automatic Runs

Perform Automatic Runs in a Standalone Mode

Perform Automatic Runs under Empower Software Control

Perform Automatic Runs under Masslynx Control

6 Create Methods, Sample Sets, and Sample Templates

Create and Edit Separation Methods

Set Separation Method Parameters

Create and Edit a Sample Set

Create a Sample Template

7 Maintenance

Maintenance Considerations

Maintain the Solvent Management System

Maintain the Sample Management System

Safety and Handling

8 Diagnostic Testing and Troubleshooting

Use the Error Log

Perform Main Diagnostic Tests

Perform Other Diagnostic Tests and Functions

Troubleshoot

A Safety Advisories

Warning Symbols

Caution Advisory

Warnings that Apply to All Waters Instruments and Devices

Warnings that Address the Replacing of Fuses

Electrical and Handling Symbols

B Specifications

Physical Specifications

Environmental Specifications

Electrical Specifications

Solvent Management Specifications

Sample Management Specifications

Instrument Control and Communication Specifications

C Spare Parts

Solvent Management System Spare Parts

Sample Management System Spare Parts

Separations Module Spare Parts

Vials and Low Volume Inserts

D Solvent Considerations

Introduction

Solvent Compatibility

Solvent Miscibility

Buffered Solvents