Solvent Blending; Prime/Vent Valve; Plunger Seal-Wash System - Waters Alliance e2695 Operator's Manual

Separation module
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1 Getting started with the Alliance e2695 Separations Module
4.
Just before the accumulator chamber empties, the solvent in the
primary piston chamber is precompressed to a pressure slightly less
than that indicated by the system pressure transducer.
5.
When the accumulator piston chamber is empty, the primary piston
delivers solvent, under pressure, through the primary pressure
transducer. It refills the accumulator piston chamber and delivers
solvent, under pressure, through the system pressure transducer,
maintaining a constant flow through the system. The cycle repeats,
beginning at
6.
The system pressure transducer measures the operating pressure. The
software compares the primary head pressure with the system pressure
and regulates the precompression step, balancing the pressures and
providing a smooth, ripple-free flow.
7.
Solvent flows from the system pressure transducer outlet to the
prime/vent valve and into an in-line filter.
8.
From the in-line filter, the solvent flows to the sample management
system.

Solvent blending

The gradient proportioning valve (GPV) blends as many as four solvents in
any combination and proportion. It produces predictable gradient segments
regardless of solvent compressibility and system backpressure. Solvent
selection and proportioning take place on the low-pressure (intake) side of the
solvent delivery system. Solvents continue to blend in each of the piston
chambers.

Prime/vent valve

The prime/vent valve is designed so that you can attach a syringe to it and
draw solvent through the solvent management system.

Plunger seal-wash system

The seal-wash solvent lubricates the plunger and flushes away any solvent or
dried salts forced past the plunger seal from the high pressure side of each
piston chamber. Washing the seals extends their life. The plunger seal wash
system operates in this sequence:
28
July 29, 2013, 715003794 Rev. B
step
3.

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