Solvent Compatibility; Solvents To Avoid - Waters Alliance e2695 Operator's Manual

Table D–1: Recommended plunger seal and seal wash solution to be used for
GPC solvents: (continued)
GPC solvent
N,N-dimethylformamide
c
(DMF)
N-methyl-2-pyrrolidone
c
(NMP)
Ortho-dichlorobenzene
(o-DCB)
Tetrahydrofuran (THF)
Toluene
1,2,4 Trichlorobenzene
e
(TCB)
f
Water
a. Package contains two seals, PN: WAT270938.
b. Package contains two seals, PN: 700001326.
c. This solvent is typically used with an additive of 0.05M lithium chloride or lithium bromide.
d. HFIP is typically used with an additive of 0.05M sodium trifluoroactetate.
e. Remove installed seal wash seals (if any) to reduce risk of premature plunger-seal failure.
f. Most aqueous GPC separations use a salt or buffer.
To prevent premature plunger seal failure when using toluene, o-dichlorobenzene (o-DCB), or
1,2,4 trichlorobenzene (TCB), Waters recommends the use of the optional Alliance Clear Seals,
the removal of installed seal wash seals, and no seal wash solvent. The use of graphite-filled
PTFE (black) seals is not recommended when any salts are present in the solvent.
D.2

Solvent compatibility

The separations module is constructed of high-quality (316) stainless steel components that, with
some minor restrictions, can be used with all solvents. This section lists the solvents that have
and have not been approved for use with the separations module.
D.2.1

Solvents to avoid

The following solvents attack or dissolve the Teflon AF tubing in the in-line vacuum degasser:
• All perfluorosolvents produced by 3M under the trade name Fluorinert
• All perfluorosolvents produced by Ausimont under the trade names Galden and Fomblin
a
With standard seal
Fluoroloy G
(yellow)
Do not use
Do not use
Not recommended
Strongly
recommended
Not recommended
Not recommended
Recommended
February 27, 2018, 715003794 Rev. C
Page 225
b
With optional seal
Alliance Clear Seal
(opaque)
Strongly
recommended
Strongly
recommended
Recommended
Strongly
recommended
Recommended
Recommended
Recommended
Seal wash
solution
50:50
methanol/water
50:50
methanol/water
None
50:50
methanol/water
None
None
20:80
methanol/water