Loss Of Prime Protection; Preferred Plunger Stroke Volume; In-Line Vacuum Degasser - Waters Alliance e2695 Operator's Manual

Separation module
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Plunger seal wash solvent flows from a reservoir to the solenoid wash
pump and then to a cavity behind the main plunger seal in the primary
head.
The solvent flows from the head and into the cavity behind the plunger
seal, in the accumulator head.
From the accumulator head, the solvent flows to waste.
When the solvent management system is delivering solvent, the plunger seal
wash pump intermittently circulates the wash solvent according to a time that
you specify.

Loss of prime protection

When a loss of prime occurs because a solvent reservoir empties, the resultant
loss of operating pressure to below 172 kPa (1.7 bar, 25 psi) for 50 cycles of the
solvent delivery mechanism causes the separations module to stop operating.

Preferred plunger stroke volume

The separations module's discrete, programmable stroke volumes provide
optimal flow delivery and solvent blending. Nevertheless, you can improve
performance for specific applications by overriding the default
(preprogrammed) stroke volume of 130 µL. To do so, you select a preferred
stroke volume of 25 µL, 50 µL, or 100 µL, as shown in the following table. A
larger stroke volume can be more effective with solvents that require
additional mixing, like trifluoroacetic acid [TFA] gradients. This additional
mixing, unlike static or dynamic mixing, occurs without added delay volume.
Alternate stroke volumes:
Flow range (mL/min)
0.050 - 0.530
0.531 - 1.230
1.231 - 3.030
3.031 - 5.000

In-line vacuum degasser

The design of the PerformancePlus in-line vacuum degasser combines a
variable speed, continuously operating vacuum pump with
Solvent management system overview
Stroke volume (µL)
25
50
100
130
July 29, 2013, 715003794 Rev. B
29

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