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Caution:
• To avoid damaging the plunger seals, run System Prep only when
solvent is in the solvent management system's flow path. If
necessary, fill all solvent reservoirs and dry prime the system before
you run System Prep.
• To avoid precipitating salts in the separations module, use an
intermediate solvent such as water when you change from buffers to
solvents of high organic content. See
page 297
To run System Prep:
1.
From either the Main screen or the Direct Functions list, select System
Prep and press Enter.
2.
In the System Prep dialog box, specify initial settings for the flow rate,
composition, sample temperature, and column temperature, and then
press the Next screen key.
3.
Select Solvents, and then press the Next screen key.
4.
Verify that the solvent reservoirs are full and solvent lines wet, and then
press the Start screen key.
Result:
separation method, putting the separations module in a state of
complete readiness for running samples. The run ends with these
results:
•
The solvent management system is primed, equilibrated, and the
column conditioned.
•
If you ran System Prep from the Direct Function list or Main screen,
the separations module enters the idle mode.
•
If you ran System Prep in a sample set, the next sample set function
determines the next phase of operation for the separations module.
Wet prime the system
Wet prime the system when you want to change reservoirs or solvents or if the
separations module has been idle for some time. Wet priming replaces solvent
in the flow path from the reservoirs to V3 (waste). If the solvent lines are dry,
dry prime before continuing (see
for information on solvent miscibility.
The System Prep run performs the steps required by your
"Solvent miscibility" on
"Dry prime the system" on page
July 29, 2013, 715003794 Rev. B
Perform direct functions
126).
129
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Table of Contents
Troubleshooting
