Water; Buffers; Tetrahydrofuran (Thf); July 29, 2013, 715003794 Rev. B - Waters Alliance e2695 Operator's Manual

Water

Use water only from a high-quality water purification system. If the water
system does not provide filtered water, filter it through a 0.45-µm membrane
filter before use.

Buffers

When you use buffers, dissolve salts first, adjust the pH, then filter to remove
insoluble material.

Tetrahydrofuran (THF)

When using unstabilized THF, ensure that your solvent is fresh. Previously
opened bottles of THF contain peroxide contaminants, which cause baseline
drift.
Warning:
concentrated or taken to dryness.

GPC solvents and seal choices

For GPC separations, see the following table for plunger seal and seal wash
solution recommendations. If required, use an intermediate solution to
prevent immiscibility or precipitation problems when you switch between
GPC and reversed-phase analytical solvents.
Recommended plunger seal and seal wash solution to be used for GPC
solvents:
GPC Solvent
Chloroform
Dimethylsulfoxide
(DMSO)
THF contaminants (peroxides) are potentially explosive if
With standard
a
seal
Fluoroloy G
(yellow)
Recommended
Recommended
With optional
b
seal
Seal wash
Alliance Clear
solution
Seal
(opaque)
Recommended 100% methanol
Recommended 100% methanol

July 29, 2013, 715003794 Rev. B

Introduction
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